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Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen*

Overview of attention for article published in Journal of Biological Chemistry, April 2016
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Title
Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen*
Published in
Journal of Biological Chemistry, April 2016
DOI 10.1074/jbc.m115.702001
Pubmed ID
Authors

Rachel Groeme, Sabi Airouche, David Kopečný, Judith Jaekel, Martin Savko, Nathalie Berjont, Laetitia Bussieres, Maxime Le Mignon, Franck Jagic, Petra Zieglmayer, Véronique Baron-Bodo, Véronique Bordas-Le Floch, Laurent Mascarell, Pierre Briozzo, Philippe Moingeon

Abstract

Allergy to the short ragweed (Ambrosia artemisiifolia) pollen is a major health problem. The ragweed allergen repertoire has been recently expanded with the identification of Amb a 11, a new major allergen belonging to the cysteine protease family. To better characterize Amb a 11, a recombinant proform of the molecule with a preserved active site was produced in Escherichia coli, refolded and processed in vitro into a mature enzyme. The enzymatic activity is revealed by maturation, following an autocatalytic processing resulting in the cleavage of both N- and C-terminal propeptides. The 2.05 Å resolution crystal structure of pro-Amb a 11 shows an overall typical C1A cysteine protease fold, with a network of molecular interactions between the N-terminal propeptide and the catalytic triad of the enzyme. The allergenicity of Amb a 11 was confirmed in a murine sensitization model, resulting in airway inflammation, production of serum IgEs as well as induction of Th2 immune responses. Of note, inflammatory responses were higher with the mature form, demonstrating that the cysteine protease activity critically contributes to the allergenicity of the molecule. Collectively, our results clearly demonstrate that Amb a 11 is a bona fide cysteine protease exhibiting a strong allergenicity. As such, it should be considered as an important molecule for diagnosis and immunotherapy of ragweed pollen allergy.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 22%
Student > Bachelor 6 19%
Researcher 4 13%
Student > Master 3 9%
Student > Doctoral Student 2 6%
Other 1 3%
Unknown 9 28%
Readers by discipline Count As %
Immunology and Microbiology 6 19%
Biochemistry, Genetics and Molecular Biology 5 16%
Chemistry 3 9%
Agricultural and Biological Sciences 3 9%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Other 3 9%
Unknown 10 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2017.
All research outputs
#15,094,401
of 25,377,790 outputs
Outputs from Journal of Biological Chemistry
#73,183
of 85,237 outputs
Outputs of similar age
#157,149
of 313,417 outputs
Outputs of similar age from Journal of Biological Chemistry
#167
of 404 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 85,237 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,417 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 404 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.