Harvey also found that SP4 is perfectly suited for removing detergents (including SDS, present in the 5-detergent SP3 lysis buffer), and even works with the recently described ‘SPEED’ protocol (lysis & precipitation in 100% TFA). https://t.co/dj2Ykkar6
@ProtifiLlc @YishaiLevin I’ve been aching to try out the S-traps, but I probably won’t be able to until my project gets funded. I’m also not convinced it will work to extract tardigrades- I am currently using a modified version of the SPEED extraction: htt
RT @PastelBio: Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics…
RT @PastelBio: Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics…