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Attention Score in Context
| Title |
Participation of Smad2, Smad3, and Smad4 in Transforming Growth Factor β (TGF-β)-induced Activation of Smad7 THE TGF-β RESPONSE ELEMENT OF THE PROMOTER REQUIRES FUNCTIONAL Smad BINDING ELEMENT AND E-BOX SEQUENCES FOR TRANSCRIPTIONAL REGULATION*
|
|---|---|
| Published in |
Journal of Biological Chemistry, September 2000
|
| DOI | 10.1074/jbc.m003282200 |
| Pubmed ID | |
| Authors |
Marcin Stopa, Dirk Anhuf, Lara Terstegen, Petros Gatsios, Axel M. Gressner, Steven Dooley |
| Abstract |
Smad7 has recently been identified as a player that antagonizes transforming growth factor beta (TGF-beta) signals by acting downstream of TGF-beta receptors. TGF-beta rapidly induces expression of Smad7 mRNA in a variety of cell types, suggesting participation in a negative feedback loop to control TGF-beta responses. We have previously described the genomic locus of rat Smad7 including the promoter region. Here we report polymerase chain reaction cloning of the corresponding promoter regions of human and murine Smad7 genes and functional characterization of the rat Smad7 promoter. Using transient transfection experiments of HepG2 cells, we identified the TGF-beta response element within a strongly conserved region, containing a perfect Smad binding element (SBE; GTCTAGAC). Performing electrophoretic mobility shift assay and cotransfection experiments, we were able to delineate DNA-binding complexes and identified Smad3, Smad4, and Smad2. Mutation of the SBE completely abolished TGF-beta inducibility of Smad7 in HepG2 cells, indicating that this sequence is necessary for TGF-beta-induced transcription. Furthermore, a 3-base pair adjacent E-box is additionally essential for TGF-beta-dependent promoter activation and an overlapping AP1 site is also involved. We conclude that regulation of Smad7 transcription by TGF-beta is mediated via a specific constellation of recognition motifs localized around the SBE, which is conserved in human, rat, and murine genes. |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Singapore | 2 | 4% |
| Canada | 1 | 2% |
| Unknown | 48 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 24% |
| Student > Ph. D. Student | 11 | 22% |
| Student > Bachelor | 4 | 8% |
| Student > Doctoral Student | 4 | 8% |
| Student > Master | 4 | 8% |
| Other | 10 | 20% |
| Unknown | 6 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 41% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Medicine and Dentistry | 5 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
| Immunology and Microbiology | 2 | 4% |
| Other | 6 | 12% |
| Unknown | 7 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2024.
All research outputs
#5,447,195
of 25,374,917 outputs
Outputs from Journal of Biological Chemistry
#13,968
of 85,240 outputs
Outputs of similar age
#6,308
of 37,744 outputs
Outputs of similar age from Journal of Biological Chemistry
#114
of 788 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 85,240 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 37,744 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 788 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.