↓ Skip to main content

ASBMB

Article Metrics

Structures of N-Glycans ofBothropsvenoms revealed as molecular signatures that contribute to venom phenotype in viperid snakes.

Overview of attention for article published in Molecular and Cellular Proteomics, May 2018
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#10 of 2,235)
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

news
8 news outlets
twitter
10 tweeters

Readers on

mendeley
3 Mendeley
Title
Structures of N-Glycans ofBothropsvenoms revealed as molecular signatures that contribute to venom phenotype in viperid snakes.
Published in
Molecular and Cellular Proteomics, May 2018
DOI 10.1074/mcp.ra118.000748
Pubmed ID
Authors

Débora Andrade-Silva, David Ashline, Thuy Tran, Aline S. Lopes, Silvia Regina Travaglia Cardoso, Marcelo S. Reis, André Zelanis, Solange M T Serrano, Vernon N. Reinhold, Aline Soriano Lopes, Marcelo da Silva Reis, Solange M. T. Serrano, Vernon Reinhold

Abstract

The complexity of snake venoms has long been investigated to explore a myriad of biologically active proteins and peptides that are used for immobilizing or killing prey, and are responsible for the pathological effects observed upon envenomation. Glycosylation is the main post-translational modification (PTM) of viperid venoms but currently there is little understanding of how protein glycosylation impacts the variation of venom proteomes. We have previously reported that Bothrops venom glycoproteomes contain a core of components that markedly define their composition and parallel their phylogenetic classification. Here we extend those observations to eight Bothrops species evaluating the N-glycomes by LC-MS as assigned cartoon structures and detailing those structures separately as methylated analogs using ion-trap mass spectrometry (MSn). Following ion disassembly through multiple steps provided sequence and linkage isomeric details that characterized 52 unique compositions in Bothrops venoms. These occurred as 60 structures, of which 26 were identified in the venoms of the Jararaca Complex (B. alcatraz, B. insularis, and B. jararaca), 20 in B. erythromelas, B. jararacussu, B. moojeni and B. neuwiedi venoms, and 22 in B. cotiara venom. Furthermore, quantitative analysis of these N-glycans showed variable relative abundances in the venoms. For the first time a comprehensive set of N-glycan structures present in snake venoms are defined. Despite the fact that glycosylation is not template-defined, the N-glycomes of these venoms mirror the phylogeny cladograms of South American bothropoid snakes reported in studies on morphological, molecular data and feeding habits, exhibiting distinct molecular signatures for each venom. Considering the complexity of N-glycan moieties generally found in glycoproteins, characterized by different degrees of branching, isomer structures, and variable abundances, our findings point to these factors as another level of complexity in Bothrops venoms, features that could dramatically contribute to their distinct biological activities.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 33%
Student > Doctoral Student 1 33%
Professor > Associate Professor 1 33%
Readers by discipline Count As %
Chemistry 2 67%
Unspecified 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 61. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2018.
All research outputs
#217,081
of 11,787,076 outputs
Outputs from Molecular and Cellular Proteomics
#10
of 2,235 outputs
Outputs of similar age
#11,284
of 258,349 outputs
Outputs of similar age from Molecular and Cellular Proteomics
#2
of 35 outputs
Altmetric has tracked 11,787,076 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,235 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,349 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.